Inkjet-based Dna Injector for Microchip Electrophoresis
نویسندگان
چکیده
We developed an inkjet-based DNA injector for microchip electrophoresis system based on a straight microchannel. The inkjet-based DNA injector enables us to precisely control injection volume of DNA and to use a simple straight microchannel for chip electrophoresis, whereas we need cross channel injector or T injector as well as complicated voltage programming for conventional chip electrophoresis. In this paper, microchip electorophoresis with inkjet injection (MCE-wii) for DNA separation was demonstrated. MCE-wii could reduce the sample volume by 0.1 % compared to that of the conventional cross injection.
منابع مشابه
Pneumatic Microvalve-Based Hydrodynamic Sample Injection for High-Throughput, Quantitative Zone Electrophoresis in Capillaries
A hybrid microchip/capillary electrophoresis (CE) system was developed to allow unbiased and lossless sample loading and high-throughput repeated injections. This new hybrid CE system consists of a poly(dimethylsiloxane) (PDMS) microchip sample injector featuring a pneumatic microvalve that separates a sample introduction channel from a short sample loading channel, and a fused-silica capillary...
متن کاملExperimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip
This study presents an experimental and numerical investigation on the use of high-resolution injection techniques to deliver sample plugs within a capillary electrophoresis (CE) microchip. The CE microfluidic device was integrated into a U-shaped injection system and an expansion chamber located at the inlet of the separation channel, which can miniize the sample leakage effect and deliver a h...
متن کاملMicrochip-based Rapid Identification of Bacillus Anthracis in Portable Gel Electrophoresis Device
In this paper, rapid identification of Bacillus anthracis genetic material in a portable microchip device for gel electrophoresis is presented. DNA fragments of virulence genes: cap, pag and sap of B. anthracis were separated and detected in a glass microchip within 4 minutes. Developed CCD-based detection setup, utilizing smart image processing, provided sensitive real-time detection of target...
متن کاملCapillary and microchip electrophoresis for rapid detection of known mutations by combining allele-specific DNA amplification with heteroduplex analysis.
BACKGROUND Detection of mutations by gel electrophoresis and allele-specific amplification by PCR (AS-PCR) is not easily scaled to accommodate a large number of samples. Alternative electrophoretic formats, such as capillary electrophoresis (CE) and microchip electrophoresis, may provide powerful platforms for simple, fast, automated, and high-throughput mutation detection after allele-specific...
متن کاملRapid prototyping of paper-based microfluidics with wax for low-cost, portable bioassay.
Here we present a simple and low-cost production method to generate paper-based microfluidic devices with wax for portable bioassay. The wax patterning method we introduced here included three different ways: (i) painting with a wax pen, (ii) printing with an inkjet printer followed by painting with a wax pen, (iii) printing by a wax printer directly. The whole process was easy to operate and c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008